European Epidermal Barrier Research Network

European Epidermal Barrier Research Network

European Epidermal Barrier Research Network

European Epidermal Barrier Research Network

Research Focus

  1. Modelling non-tumoral epidermal pathologies in vitro: inflammatory, genetic and infectious diseases

  2. Epidermal morphogenesis and senescence keratinocytes

  3. Cell and tissue response in reconstructed human epidermis after application of irritant or sensitizer chemicals



Research Focus Picture


Important Publications

  1. Faway, E., Cambier, L., De Vuyst, E., Evrard, C., Thiry, M., Lambert de Rouvroit, C., Mignon, B. & Poumay, Y. (2019) Responses of reconstructed human epidermis to Trichophyton rubrum infection and impairment of infection by the inhibitor PD169316. Article in press in the J. Invest. Dermatol. doi.org/10.1016/j.jid.2019.03.1147

  2. Faway, E., Lambert de Rouvroit, C. & Poumay, Y. (2018) In vitro models of dermatophyte infection to investigate epidermal barrier alterations. Exp. Dermatol. 27, 915-922. doi: 10.1111/exd.13726

  3. De Vuyst, E., Salmon, M., Evrard, C., Lambert de Rouvroit, C. & Poumay, Y. (2017) Atopic dermatitis studies through in vitro models. Frontiers in Medicine, 4, article 119. doi: 10.3389/fmed.2017.00119

  4. Mound, A., Warnon, C., Lozanova, V., Hermant, M., Robic, J., Guere, C., Vie, K., Lambert de Rouvroit, C., Tyteca, D., Debacq-Chainiaux, F. & Poumay, Y. (2017) Non-senescent keratinocytes organize sphingomyelin-rich lipid submicrometric domains in plasma membrane. BBA-Molecular and Cell Biology of Lipids, 1862, 958-971. Doi: 10.1016/j.bbalip.2017.06.001

  5. Do Nascimento Pedrosa, T., De Vuyst, E., Mound, A., Lambert de Rouvroit, C., Stuchi Maria-Engler, S. & Poumay, Y. (2017) Plasma membrane cholesterol depletion combined with interleukin-4 reproduces major features of atopic dermatitis in a 3D-culture model. Arch. Dermatol. Res., 309, 63-69. Doi : 10.1007/s00403-016-1699-7.

  6. De Vuyst, E., Mound, A., Lambert de Rouvroit, C. & Poumay, Y. (2016) Modelling atopic dermatitis during the morphogenetic process involved in reconstruction of the human epidermis. Current Research in Translational Medicine. 64, 179-183. Doi: 10.1016/j.retram.2016.10.005 (review)

  7. Faway, E., Cambier, L., Lambert de Rouvroit, C., Mignon, B. & Poumay, Y. (2016) Modeling dermatophytosis in reconstructed human epidermis: a new tool to study infection mechanisms and to test antifungal agents. Medical Mycology 55, 485-494. Doi: 10.1093/mmy/myw111

  8. De Vuyst, E., Giltaire, S., Lambert de Rouvroit, C., Malaisse, J., Mound, A., Bourtembourg, M., Nikkels, A.F., Chrétien, A., Salmon, M. & Poumay, Y. (2016) MßCD concurs with IL-4, IL-13 and IL-25 to induce alterations reminiscent of atopic dermatitis in reconstructed human epidermis. Exp. Dermatol. 27 (4), 435-437. Doi: 10.1111/exd.13113

  9. Hayez, A., Roegiers, E., Malaisse, J., Achouri, Y., Lambert de Rouvroit, C., Poumay, Y., Michiels, C. & De Backer, O. (2016) TMEM45A is dispensable for epidermal morphogenesis, keratinization and barrier formation. PLoS ONE, 11(1):e0147069. doi: 10.1371/journal.pone.0147069.

  10. Malaisse, J., Pendaries, V., Hontoir, F., De Glas, V., Van Vlaender,D., Simon, M., Lambert de Rouvroit, C., Poumay, Y. & Flamion, B. (2016) Hyaluronan does not regulate human epidermal keratinocyte proliferation and differentiation. J. Biol. Chem. 291, 6347-6358. doi : 10.1074/jbc.M115.661348.

  11. Mound, A., Malaisse, J., De Vuyst, E., Hayez, A., Lambert de Rouvroit, C., Pittelkow, M.R. & Poumay, Y. (2015) Epidermal reference genes at the forefront of data interpretation. Exp. Dermatol. 24, 738-739. Doi: 10.11211/exd.12811

  12. Malaisse, J., Evrard, C., Feret, D., Colombaro, V., Dogné, S., Haftek, M., Lambert de Rouvroit, C., Flamion, B. & Poumay, Y. (2015) Hyaluronidase-1 is mainly functional in the upper granular layer, close to the epidermal barrier. J. Invest. Dermatol. 135, 3189-3192. doi: 10.1038/jid.2015.299

  13. Aqil, A., Tchemtchoua, V.T., Colige, A., Atanasova, G., Poumay, Y. & Jérôme, C. (2015) Preparation and characterizations of EGDE crosslinked chitosan electrospun membranes. Clinical Hemorheology and Microcirculation. 60, 39-50. doi: 10.3233/CH-151930

  14. De Vuyst, E., Giltaire, S., Lambert de Rouvroit, C., Chrétien, A., Salmon, M. & Poumay, Y. (2015) The activation of cultured keratinocytes by cholesterol depletion during reconstruction of a human epidermis is reminiscent of monolayer cultures. Arch. Dermatol. Res. 307, 309-318 DOI: 10.1007/s00403-015-1537-3

  15. Croisier, F., Atanasova, G., Poumay, Y. & Jérôme, C. (2014) Polysaccharide-coated PCL nanofibers for wound dressing applications. Advanced Healthcare Materials 3(12): 2032-2039. doi: 10.1002/adhm.201400380

  16. Malaisse, J., Hermant, M., Hayez, A., Poumay, Y. & Lambert de Rouvroit, C. (2014) Meaning of relative gene expression in multilayered cultures of epidermal keratinocytes. Exp. Dermatol. 23 :754-756. DOI:10.1111/exd.12501

  17. Hayez, A., Malaisse, J., Roegiers, E., Reynier, M., Renard, C., Haftek, M., Geenen, V., Serre, G., Simon, M., Lambert de Rouvroit, C., Michiels, C. & Poumay, Y. (2014) High TMEM45A expression is correlated to epidermal keratinization. Exp. Dermatol. 23, 339-344. DOI: 10.1111/exd.12403

  18. Malaisse, J., Bourguignon, V., De Vuyst, E., Lambert de Rouvroit, C., Nikkels, A.F., Flamion, B. & Poumay, Y. (2014) Hyaluronan metabolism in human keratinocytes and atopic dermatitis skin is driven by a balance of hyaluronan synthases 1 and 3. J. Invest. Dermatol. Published online. Doi: 10.1038/jid.2014.147

  19. Lambert de Rouvroit, C., Charlier, C., Lederer, D., De Glas, V., De Vuyst, E., Dargent, J.L., Grammatico, P., Binni, F., Rousseau, C., Hennecker, J.L., Nikkels, A.F. & Poumay, Y. (2013) In vitro reconstruction of epidermis from primary Darier’s disease keratinocytes replicates the histopathological phenotype. J. Dermatol. Sci. 71, 138-140. doi:10.1016/j.jdermsci.2013.04.016

  20. Flamant, L., Roegiers, E., Pierre, M., Hayez, A., Sterpin, C., De Backer, O., Arnould, T., Poumay, Y. & Michiels, C. (2012) TMEM45A as a new biomarker of chemoresistance in cancer cells. BMC Cancer 12:391. doi: 10.1186/1471-2407-12-391

  21. Frankart, A., Malaisse, J., Minner, F., De Vuyst, E., Lambert de Rouvroit, C. & Poumay, Y. (2012) Epidermal morphogenesis during progressive in vitro 3D reconstruction at the air-liquid interface. Exp. Dermatol. 21, 871-875. DOI:10.1111/exd.12020

  22. Frankart, A., Coquette, A., Schröder, K.R. & Poumay, Y. (2012) EGF receptor signalling is induced by sensitizing chemicals in keratinocytes of reconstructed human epidermis and regulates the release of interleukin-8.Arch. Dermatol. Res. 304, 289-303. DOI: 10.1007/s00403-012-1209-5

  23. Giltaire, S., Lambert, S. & Poumay, Y. (2011) HB-EGF synthesis and release induced by cholesterol depletion of human epidermal keratinocytes is controlled by extracellular ATP. J. Cell. Physiol. 226, 1651-1659. DOI: 10.1002/jcp.22496

  24. Mathay, C., Pierre, M., Pittelkow, M.R., Depiereux, E., Nikkels, A.F., Colige, A. & Poumay, Y. (2011) Transcriptional profiling after lipid raft disruption in keratinocytes identifies critical mediators of atopic dermatitis pathways. J. Invest. Dermatol. 131, 46-58. doi:10.1038/jid.2010.272

  25. Mathay, C. & Poumay, Y. (2010) Lipid rafts and the oxidative stress hypothesis. J. Invest. Dermatol. 130, 1457-1459. doi:10.1038/jid.2009.425

  26. Minner, F. & Poumay, Y. (2009) Candidate house-keeping genes require evaluation before their selection for studies of human epidermal keratinocytes. J. Invest. Dermatol, 129, 770-773. doi:10.1038/jid.2008.247

  27. Giltaire, S., Herphelin, F., Hérin, M., Stoppie, P. & Poumay, Y. (2009) The CYP26 inhibitor R115866 potentiates the effects of all-trans-retinoic acid on cultured human epidermal keratinocytes. Br. J. Dermatol., 160, 505-513. doi 10.1111/j.1365-2133.2008.08960.x

  28. Mathay, C., Giltaire, S, Minner, F., Bera, E., Hérin, M. & Poumay, Y. (2008) Heparin-binding EGF-like growth factor is induced by disruption of lipid rafts and oxidative stress in keratinocytes and participates in the epidermal response to cutaneous wounds. J. Invest. Dermatol., 128(3), 717-727. doi:10.1038/sj.jid.5701069

  29. Lambert, S., Ameels, H., Gniadecki, R., Hérin, M. & Poumay, Y. (2008) Internalization of EGF receptor following lipid rafts disruption in keratinocytes is delayed and dependent on p38 MAPK activation. J. Cell. Physiol., 217, 834-845. DOI: 10.1002/jcp.21563

  30. Poumay, Y. & Coquette, A. (2007) Modelling the human epidermis in vitro: tools for basic and applied research. Arch. Dermatol. Res. 298, 361-369. DOI:10.1007/s00403-006-0709-6

  31. Poumay, Y., Dupont, F., Marcoux, S., Leclercq-Smekens, M., Hérin, M. & Coquette, A. (2004) A simple reconstructed human epidermis : preparation of the culture model and utilization in in vitro studies. Arch. Dermatol. Res. 296, 203-211. DOI 10.1007/s00403-004-0507-y

  32. Jans, R., Atanasova, G., Jadot, M. & Poumay, Y. (2004) Cholesterol depletion upregulates involucrin expression in epidermal keratinocytes through activation of p38. J. Invest. Dermatol. 123, 564-573. DOI : 10.1111/j.0022-202x.2004.23221.x

  33. Jans, R., Sartor, M., Jadot, M. & Poumay, Y. (2004) Calcium entry into keratinocytes induces exocytosis of lysosomes. Arch. Dermatol. Res. 296, 30-41. DOI: 10.1007/s00403-004-0469-0

  34. Coquette, A., Berna, N., Vandenbosch, A., Rosdy, M., De Wever, B., & Poumay, Y. (2003) Analysis of the induction and release of interleukin-1a (IL-1a) and interleukin-8 (IL-8) in in vitro reconstructed human epidermis in order to predict in vivo skin irritation and/or sensitisation. Toxicology In Vitro 17, 311-321

  35. De Potter, I.Y., Poumay, Y., Squillace, K. & Pittelkow, M.R. (2001) Human EGF Receptor (HER) family and Heregulins members are differentially expressed in epidermal keratinocytes and modulate differentiation. Exp. Cell Res. 271, 315-328

  36. Smits, P., Poumay, Y., Tylzanowski, P., Karperien, M., Wouters, J., Ponec, M., Huylabroeck, D. & Merregaert, J. (2000) Differentiation-dependent alternative splicing and expression of the Extracellular Matrix Protein 1 (Ecm1) gene in human keratinocytes. J. Invest. Dermatol. 114, 718-724

  37. Baudoux, B., Castanares-Zapatero, D., Leclercq-Smekens, M, Berna, N.. & Poumay, Y. (2000) The tetraspanin CD9 associates with the integrin a6b4 in cultured human epidermal keratinocytes and is involved in cell motility. Eur. J. Cell Biol. 79, 41-51

last update: 2019, December